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| Three-Dimensional Organization of Actin Filaments in Dividing Cells |
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| D.J. Fishkind and Y.-l. Wang (1993) |
| J. Cell Biol.123:837-848. |
| It is known that cleavage forces are generated through actin-myosin interactions. To study the organization of actin filaments, dividing Normal Rat Kidney cells were stained with fluorescent phalloidin and imaged with digital optical sectioning microscopy. Optical sections were then processed with the nearest neighbor deconvolution algorithm and reconstructed into perspective views at different angles. |
| Reconstructed Views of Actin Filaments during Cytokinesis |
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Size = 304 x 216 pixels.
Size = 304 x 216 pixels.
Size = 304 x 216 pixels.| Equatorial organization of actin filaments is found primarily along the equator of the bottom cortex, where cleavage is constrained by cell-substrate adhesions. The top cortex, where the cell cleaves actively, shows a limited organization of actin filmanets. No "purse string" around the equator can be found. Many actin filaments in the region flanking the equator are oriented along the long axis of the cell. This organization suggests that the "purse string" model of cleavage may not apply to cultured mammalian cells and that ripping forces along opposite directions of the long axis may play a role. |
| Organization of Actin Filaments in the Top Half of the Cell |
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Size = 304 x 216 pixels.| Organization of Actin Filaments in the Bottom Half of the Cell |
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Size = 304 x 216 pixels.