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| Probing the Role of Myosin Light Chain Kinase in Cytokinesis |
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| D.J. Fishkind, L.-g. Cao and Y.-l. Wang (1991) |
| J. Cell Biol. 114:967-975. |
| At least part of the cleavage forces are generated by myosin II, which is turned on by the calcium-calmodulin regulated myosin light chain kinase. To investigate the functional role of this regulatory mechanism, we have microinjected a fragment of myosin light chain kinase that is no longer regulated by calcium-calmodulin. |
| Microinjection of Unregulated Myosin Light Chain Kinase Induces Membrane Blebbing but Has No Effect on Cell Cleavage |
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Size = 136 x 188 pixels.
Size = 136 x 136 pixels.| Active fragments of myosin light chain kinase cause blebbing of the membrane in regions outside the cleavage furrow. The blebbing activity may represent disorganized cortical contractions. However cells divide normally. Thus it is possible that myosin light chain kinase is selectively activated in the cleavage furrow, but this is not the primary switch defining the timing and location of cell division. |