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| Effects of ATP Depletion on Actin Organization |
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| Y.-l. Wang (1986) |
| Exp. Cell Res. 167:16-28. |
| To study how energy depletion affects actin organization, 3T3 fibroblasts were microinjected with fluorescently labeled alpha-actinin, which incorporates into actin filament bundles, lamellipodia, and focal adhesions. Cells were then treated with a combination of sodium azide and deoxyglucose to deplete ATP. |
| Collapse of Stress Fibers upon ATP Depletion |
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Size = 280 x 236 pixels.| ATP deplection causes an immediately retraction of all the lamellipodia. Actin filament bundles (stress fibers) collapse into tight aggregates over 1-2 hours. |
| Recovery of Stress Fibers upon Drug Removal |
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Size = 320 x 220 pixels.| Upon the removal of sodium azide and deoxyglucose, lamellipodia reappear transiently in the same region where they retracted. Aggregates of collapsed stress fibers extend back into long bundles. |
| Recovery of Stress Fibers at A High Magnification |
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Size = 320 x 240 pixels.| At high magnifications, the recovery of stress fibers appears to involve telescoping of filaments into long bundles. |