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| CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis |
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| S.P. Wheatley, E.H. Hinchcliffe, M. Glotzer, G. Sluder and Y.-l. Wang (1997) |
| J. Cell Biol. 138:385-393. |
| CDK1 functions as a master regulator of mitosis. It is activated by cyclin B upon the entry into mitosis and inactivated during anaphase onset with the degradation of cyclin B. To identify events during anaphase and telophase that depend on CDK1 inactivation, we expressed a non-degradable cyclin B in dividing Normal Rat Kidney cells. |
| Effects of Sustained CDK1 Activity on Chromosome Separation and Cytokinesis |
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Size = 236 x 152 pixels.
Size = 196 x 180 pixels.| Sustained CDK1 activity is induced by the microinjection of mRNA coding for a non-degradable cyclin B. In these cells chromosomes separate normally at anaphase onset, but unlike control cells the separation of chromosomes continues until chromosomes reach the distal cortex, where they often bounce back to the central region of the cell. Cytokinesis never takes place in most injected cells. |
| Effects of Sustained CDK1 Activity on Microtubule Organization |
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Size = 188 x 160 pixels.| Cells loaded with rhodamine tubulin show exaggerated spindle pole separation. In addition, interzonal microtubules never assembled in cells with sustained CDK1 activities. Compare this with the organization of microtubules in control cells. |