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| Dynamics of Actin Filaments during Cell Division |
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| L.-g. Cao and Y.-l Wang (1990) |
| J. Cell Biol. 111:1905-1911. |
| To examine the movement of actin filaments during cell division, we have microinjected fragments of fluorescently labeled actin filaments or fluorescent phalloidin into living Normal Rat Kidney cells. Injected cells were then imaged with low light level detectors during cytokinesis. |
| Movement of Microinjected Actin Filaments into the Cleavage Furrow |
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Size = 284 x 208 pixels.| Actin filaments were assembled in vitro and labeled/stabilized with rhodamine phalloidin. The filaments were sheared into short fragments before microinjection into NRK cells during early anaphase. The site of injection appeared as a large bright spot near the top of the image. During the early phase of cytokinesis, several small aggregates of actin filaments dissociated from the site of injection and moved toward the equator, which is marked by arrows. |
| Movement and Contraction of Endogneous Phalloidin-Labeled Actin Filaments during Cleavage |
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Size = 320 x 212 pixels.| A well spread, dividing NRK cell was microinjected with fluorescent phalloidin to label its actin filaments. Filament bundles are clearly visible in the cleavage furrow and radiating out. The movement of actin filament during cleavage appears to involve both constriction and tearing in opposite directions. The structure disassembles at the same time. |