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| Dynamics of Myosin II in Living Fibroblasts |
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| N.M. McKenna, Y.-l. Wang and M.E. Konkel (1989) |
| J. Cell Biol. 109:1163-1172. |
| Myosin II interacts with actin filaments to generate contractile forces. To investigate the formation of myosin contractile structures in cultured non-muscle cells, we microinjected fluorescently labeled myosin into living 3T3 fibroblasts. |
| Assembly and Rearward Transport of Myosin II Minifilaments in Motile Cells |
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Size = 252 x 220 pixels.
Size = 204 x 232 pixels.| These video sequences show the continuous formation of myosin punctate structures behind active lamellipodia, and subsequent movement toward the center of the cell. Studies from other laboratories indicate that these structures consist of small side-by-side, ribbon-like complexes of myosin molecules. These complexes most likely disassemble somewhere near the center of the cell, releasing myosin subunits back to the anterior end for assembly. |
| Organization of Myosin II Minifilaments into Lattice |
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Size = 268 x 204 pixels.| This sequence shows the organization of myosin punctate structures into a two-dimensional lattice |